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Single Mutation at a Highly Conserved Region of Chloramphenicol Acetyltransferase Enables Isobutyl Acetate Production Directly from Cellulose by Clostridium Thermocellum at Elevated Temperatures

Computer generated orange, green, blue, and yellow corkscrew shapes.

Cong Trinh and his research team discovered an engineered chloramphenicol acetyltransferase can be harnessed to create a novel thermophilic microbial platform for consolidated bioprocessing of lignocellulosic biomass into designer bioesters with broad applications as fuels, chemicals, flavors, and fragrances where saccharification, fermentation, and separation can be conducted in a single step.

This research is published in the journal of Biotechnology for Biofuels.